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0000001500 00000 n Per ulteriori informazioni leggere quanto segue ATTENZIONE: Per acquistare il prodotto è necessario il patentino in corso di validità per acquisto e uso di fitofarmaci classificati come Xn, T, T+. We bought the Glufosinate-ammonium from Sigma.What should be the concentration of Basta (Ammonium Glufosinate) to be used to screen tansgenic Arabidopsis seeds in MS Agar medium?Muhammad Sufyan Khalid, bother, I suggest you optimize the concentration of Basta for the selection of your desired transgenic plants. represents a route whereby foreign genes and their products may escape into the wider environment.
So, I tried to sterilize the seeds with NaOCl and SDS. Is it usual, or is something amiss? But colony PCR and PCR on mini preps were negative. View product specific information, MSDS and references. 10 27 by cross-hybridization with wild relatives have been extensively explored, but problems that may ari...I am working on lettuce. For this purpose, first, you should grow wild plants separately. Boroplus Valagro si distribuisce uniformemente...Vendita feromoni per insetti da utilizzare con...La dose di 3,0 l/ha è efficace contro le infestanti negli stadi giovanili di sviluppo. bioWORLD strives to remain highly competitive with respect to pricing, but all prices are subject to change with out notice. 0000001457 00000 n All quotes are valid for 30 days unless otherwise stated. Do i really need homozygous plants to achieve my objective/aim?Maybe 20 mg/L on plates is more reliable, wish you good luck!In this case, I would like to use the 1/2 MS medium plus agar and 20 g sucrose per littre. There were 6 fatalities in 34 cases of glufosinate-ammonium poisoning reported by the Japan Poison Information Center. Il ministero della Salute, con decreto del 26 aprile 2012, ha revocato la sospensione cautelativa del prodotto fitosanitario Basta® 200, erbicida di Bayer CropScience a base di glufosinate ammonio; con decreto del 27 aprile 2012 lo stesso ministero ha emanato quindi un nuovo provvedimento di ri-registrazione per il suddetto formulato che ne consente la commercializzazione in Italia fino al 30 … Thank you. Has anybody experienced something similar?What should I do if Agrobacterium keep on growing on the selection medium? Per poter concludere l'acquisto indicare nelle note dell'ordine: La quantità minima ordinabile per questo prodotto è - Cocciniglia Cotonosa solcata degli agrumi Toelettatura per cani e gatti Policoro MT Questo prodotto al momento non è disponibile- Come eliminare le Larve di Maggiolino e gli adulti - Melolontha- Farfalla delle palme Paysandisia archonBREXIL Zn VALAGRO è un concime biologico con... Con l'acquisto di questo prodotto è possibile raccogliere fino a Dichiaro di aver letto le condizioni sulla privacy.per quale motivo e' stato ritirato dal commercio?Laser è un prodotto a base di Spinosad (44 %)...Attenzione: Ultimi articoli in magazzino!- Come eliminare le formiche dall'orto, giardini e campi 0000001928 00000 n for seedling experiment if it is in vitro, you increase go up to 15-25 mg /L.
0000006732 00000 n Arabidopsis transgenic seeds were germinated on MS plates containing BASTA herbicide (15 μg/ml Glufosinate-ammonium). CAS 77182-82-2. ���|ހ��c� Questa caratteristica ne permette il contemporaneo utilizzo anche come spollonante. a 100. &�E?���pp���>�8��Hb�>,#����Eg�� �6 ��O��'��D�1��?u��cCV���C��ֆ��h�.Ϋ#s�5�լJ��ײU�e�+���������yg��W�bb4��O6�&�∼���I���O7V��>u����@B~�F̥ Glufosinate –ammonium (200g/l) Formulation type: Soluble concentrate (SL) Chemical Family: Amino-phosphonate Conditioning: 5 L gallon Mode of action: BASTA F1 acts by inhibiting glutamine synthetase. Then after discussion with your supervisor, use two different concentrations separately on wild type plants. Locate a Territory Manager. All rights reserved.Efficient selection and evaluation of transgenic lines of Crambe abyssinica After performing the transformation experiment, I have collected the seeds. Indian Institute of Science Education and Research BhopalActivities of CaMV 35S and nos promoters in pollen: Implications for field release of transgenic plantsErratum: State transitions revisited - A buffering system for dynamic low light acclimation of Arabidopsis (Plant Molecular Biology DOI: 10.1007/s11103-006-9044-8)I normally use 10 mg/L when I do selection on MS plates and it works pretty well. Can anyone tell me the appropriate concentration and brand of BASTA to be used for MS medium? 0000002719 00000 n Sorry, it was a typing error. 2. Glufosinate-ammonium is a crop protection product that works by inhibiting an enzyme central to plant metabolism. I was using 3 mg/L Bialaphos instead of 8 mg PPT. startxref glufosinate ammonium/kg bw/day equivalent to 101 ppm in the feed. Then select the best concentration on which wild type plants completely died. How many plants do i need to select from T2 for metabolomics?How can I confirm Agrobacterium (GV3101) transformation?Join ResearchGate to find the people and research you need to help your work.I am facing problems in confirmation of transformed GV3103. Any suggestion so that i can eliminate Agrobacterium without causing albinism or kill the putative transformed plant? 0000001689 00000 n trailer The possible risks posed I use Hygromycin 20mg/ml stock and Carbenicillin 400mg/ml. BASTA F1 acts by inhibiting glutamine synthetase. 0000001604 00000 n 0000000836 00000 n And should I spray more in the roots, or in the whole plant?I want to select transgenic Arabidopsis seeds using BASTA on MS medium. 10 0 obj <> endobj *�$r�)dO 0000001405 00000 n 0000001047 00000 n h�b```a``nd`e`��d�c@ >�+s,`8���p